Welcome to the Haddad Lab @
http://haddadlab.ucsd.edu
|
|
|
| Welcome to Our Lab! |
| The major thrust in our laboratory is to decipher the cellular and molecular mechanisms involved in the response, adaptation, or injury during and following O2 deprivation. For the past 20 years, we have defined and characterized the cellular and molecular alterations that can lead to survival of cells or to injury and cell death. Techniques used involve voltage and current recordings using sharp and patch electrodes (whole-cell and single channel). In addition, intracellular ionic measurements (e.g. pH, Ca++) have been made mostly using optical methods (video and confocal microscopy). More recently, we have been interested in particular in K+ channels (ATP-sensitive K+ channel and the BK channel), whether on the plasmalemma or on mitochondrial membranes, which seem to have important roles to play in O2 deprivation. In the past 10 years, we have started to use genetic/genomic approaches to study anoxia/hypoxia tolerance or susceptibility. We have embarked on the study of Drosophila melanogaster, which is very anoxia-tolerant. We have also started on a productive mutagenesis screen to obtain loss-of-function mutants. This is on going using P-elements or EP lines and we have already obtained a number of genes and families of genes that are important in O2 deprivation. A number of other approaches are also being used to understand the extraordinary tolerance of the fruit fly to lack of O2 including studies involving the evolution of genes under stressful conditions. |
|
|
|
|
|
| Contact Us |
Leichtag Building #481
MC 0735
Pat Spindler
Phone: (858) 822-4740
Fax: (858) 534-6972
|
|
|
|
|
|
|
|